Development and Application of an In-house Line Probe Assay for Hepatitis C Virus Genotyping

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development and application of an in-house line probe assay for hepatitis c virus genotyping

background hepatitis c virus (hcv) is the major cause of chronic liver disease. hcv is a single stranded positive sense rna of approximately 9.6 kb. because of high conservativeness of 5΄untranslated region of hcv genome, it is widely used for virus genotyping. different methods are used for the virus genotyping, but all involve some difficulties. objectives the aim of the present study was to ...

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Development and Application of an In-house Line Probe Assay for Hepatitis C Virus Genotyping

BACKGROUND Hepatitis C virus (HCV) is the major cause of chronic liver disease. HCV is a single stranded positive sense RNA of approximately 9.6 Kb. Because of high conservativeness of 5΄untranslated region of HCV genome, it is widely used for virus genotyping. Different methods are used for the virus genotyping, but all involve some difficulties. OBJECTIVES The aim of the present study was t...

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Second-generation line probe assay for hepatitis C virus genotyping.

Because of the enormous variability of hepatitis C virus (HCV), the development of reliable genotyping assays is a formidable challenge. The optimal genotyping region appears to be the 5' untranslated region (UR) because of high conservation within, but considerable variability between, genotypes. In this study, 21 probes dispersed over seven variable 5' UR areas were applied to a line probe as...

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Use of a commercially available line probe assay for genotyping of hepatitis C virus 5a strains.

To validate a commercially available line probe assay for samples containing the infrequently found hepatitis C virus (HCV) genotype 5a, we sequenced a 511-nucleotide fragment of the NS4b region of Belgian and South African HCV genotype 5a samples. Phylogenetic analysis of the sequence data was performed. For the 77 HCV genotype 5a samples collected, there was 100% concordance between the genot...

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ژورنال

عنوان ژورنال: Hepatitis Monthly

سال: 2013

ISSN: 1735-143X,1735-3408

DOI: 10.5812/hepatmon.6767